Kerusakan dinding sel Escherichia coli K1.1 oleh minyak atsiri temu kunci (Kaempferia pandurata) [Cell wall disruption of Escherichia coli Kdchia coli K1 1 by temu kunci (Kaeempferia pandurata) essential oil]

Miksusanti, Miksusanti and Betty Sri Laksmi Jennie, Betty Sri Laksmi Jennie and Bambang Ponco, Bambang Ponco and Gatot Tri Mulyadi, Gatot Tri Mulyadi (2008) Kerusakan dinding sel Escherichia coli K1.1 oleh minyak atsiri temu kunci (Kaempferia pandurata) [Cell wall disruption of Escherichia coli Kdchia coli K1 1 by temu kunci (Kaeempferia pandurata) essential oil]. Berita Biologi Jurnal Ilmiah Nasional, 9 (1). pp. 1-8. ISSN 0126-1754

[img]
Preview
PDF
Download (3412b) | Preview

    Abstract

    Antibacterial of temu kunci (Kaempferia padurata) essential oil against Escheria coli K1.1 cell wall and altering it. minimum inhibitory concentration (MIC) of temu kunci essential oil is 0.11% (v/v). further studies were conducted using the concentration of 1 MIC and 2 MIC. Leakage phenomena were monitored with atomic adsorption spectrometry (AAS), and ultraviolet spectrophotometry (UV). alteration of cell wall was analyzed with scanning electron microscopy (SEM). the optical density values observed by UV spectrophotometer for protein and nucleic acid leakage were 0.3813-0.6573 at 280 nm and 0.2186-0.5603 at 260 nm. the result showed that K. Pandurata essential oil could leak the inorganic ion Ca+2 17-53%, and K+1 9-43% from the bacteria and alter the cell wall of the bacteria

    Item Type: Article
    Uncontrolled Keywords: Escherichia coli K1.1, temu kunci, Kaempferia pandurata, kebocoran ion, perubahan dinding sel, protein, asam nukleat
    Subjects: Q Science > QD Chemistry
    Divisions: Faculty of Mathematics and Natural Sciences > Department of Chemistry
    Depositing User: Dr MIKSUSANTI MIKSUSANTI
    Date Deposited: 14 Sep 2016 07:34
    Last Modified: 14 Sep 2016 07:34
    URI: http://eprints.unsri.ac.id/id/eprint/6218

    Actions (login required)

    View Item