Yeast Saccharomyces cerevisiae as Model to Identify Mengkudu (Morinda citrifolia) as an Anticancer Medicinal Plants Candidates with Antiproliferative Properties

Hermansyah, Hermansyah (2012) Yeast Saccharomyces cerevisiae as Model to Identify Mengkudu (Morinda citrifolia) as an Anticancer Medicinal Plants Candidates with Antiproliferative Properties. In: The 5 Indonesia Biotechnology Conference an International Forum, 4-7 Juli 2012, Lombok, Indonesia.

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      Abstract

      Although yeast Saccharomyces cerevisiae is an excellent eukaryotic model organism, however, to use S.cerevisiae cell as a model organism screening drugs is rare. In this research, we used S.cerevisiae to screen antiproliferative properties of medicinal plants candidates such as pare (Momordica charantia L.), sukun (Artocarpus communis), and mengkudu or noni fruit (Morinda citrifolia). The results showed that 3% w/v extract of mengkudu, but not pare and sukun extracts was capable to inhibit growth of S.cerevisiae effectively. This inhibited phenotypes was confirmed by measuring growth rate of cells in cultures treated with various concentrastions of extracts. Morphologycal analysis using differential interference contrast (DIC) microscopy showed that S.cerevisiae wild type strain FY833 cells was accumulated in the unbudded cells, and further analysis using Fluorescence Activated Cell Sorting (FACS) analysis exhibited that proliferation of cells run unproperly because of cells are arrested in G1 phase, indicating that G1 to S phase transition progression of S.cerevisiae cell cycle was inhibited in the presence of mengkudu extract.

      Item Type: Conference or Workshop Item (Paper)
      Uncontrolled Keywords: Saccharomyces cerevisiae, mengkudu, anticancer antiproliferative
      Subjects: Q Science > QD Chemistry
      R Medicine > RS Pharmacy and materia medica
      T Technology > TP Chemical technology
      Divisions: Faculty of Mathematics and Natural Sciences > Department of Chemistry
      Depositing User: Rudi Kule
      Date Deposited: 12 Feb 2016 11:48
      Last Modified: 12 Feb 2016 11:48
      URI: http://eprints.unsri.ac.id/id/eprint/6142

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