Optimizing the Enzymatic Hydrolysis of Skipjack Tuna (Katsuwonus pelamis) Dark Flesh Using Alcalase Enzyme: A Response Surface Approach

Herpandi, Herpandi and Huda, N. and Rosma, A. and Nadiah, W.A. Wan (2013) Optimizing the Enzymatic Hydrolysis of Skipjack Tuna (Katsuwonus pelamis) Dark Flesh Using Alcalase Enzyme: A Response Surface Approach. Journal of Fisheries and Aquatic Science, 8 (4). pp. 494-505. ISSN 18164927

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    Abstract

    The goal of this study was to optimize enzymatic hydrolysis of the dark flesh of skipjack tuna (Katsuwonus pelamis). Alcalase 2.4 L was used as the hydrolytic enzyme at various concentrations (1, 1.5, 2, 2.5 and 3% w/w) at pHs from 6 to 10, temperatures from 35 to 75°C and times of 2 to 6 h. The experiment was performed following a five-level second-order central composite design with six replications at the center points. The high coefficients of determination for degree of hydrolysis (R2 = 0.8909) and free tryptophan content (R2 = 0.9732) indicate the suitability of the design for predicting the responses. The optimum hydrolytic conditions predicted by the response surface methodology were 2% Alcalase at pH 8.86 and 65.4°C for 5.74 h. These conditions resulted in an experimental degree of hydrolysis of 20.74% and free tryptophan content of 102.23 mg kg-1.

    Item Type: Article
    Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
    Divisions: Faculty of Agriculture > Department of Fishery Products Technology
    Depositing User: Mr Herpandi Napis
    Date Deposited: 29 Jun 2015 21:04
    Last Modified: 29 Jun 2015 21:48
    URI: http://eprints.unsri.ac.id/id/eprint/5519

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