EFFECT OF CHITOSAN ON PROTEIN CONTENT IN THE MEDIUM CULTURE OF OSTEOBLASTS EXPOSED TO OXIDATIVE STRESS

Chairani, Shanty and Utami, Sri and Suniarti, Dewi F. (2011) EFFECT OF CHITOSAN ON PROTEIN CONTENT IN THE MEDIUM CULTURE OF OSTEOBLASTS EXPOSED TO OXIDATIVE STRESS. dentika Dental Journal, 16 (1). pp. 53-56. ISSN 1693-671X

[img]
Preview
PDF - Published Version
Download (7Mb) | Preview

    Abstract

    Chitosan is a derivative of chitin which has potential for use in bone regeneration and has been reported can stimulate bone formation. Oxidative stress as one cause of bone damage, was found increased in osteoporosis, periodontitis and arthritis. One of the species oxygen reactive (ROS), hydrogen peroxide, has been reported can inhibit osteoblast pro-liferation. This study was aimed to investigate the effect of various chitosan concentrations on protein content in the culture medium of human osteoblast-like cell line, MG 63, which was exposed to hydrogen peroxide. MG 63 cells were exposed to various chitosan concentrations (% w/v) 0.1, 0.2, 0.4, and 1.6%. Culture cells without chitosan were used as a control. Cells were growth with α-MEM medium (37ºC, 5% CO2) until they became confluent, then they were exposed to hydrogen peroxide for 4 hours. The protein content in the culture medium was measured by using Bradford protein assay at 655 nm wavelength. The result showed that hydrogen peroxide decreased protein concentration in the medium culture compared with group without hydrogen peroxide. Treatment group with chitosan concentration 0.4% and 1.6% exhibited a significant increasing of protein concentration in osteoblast culture medium compared with control. In conclusion, in osteoblast culture medium chitosan can inhibit the decreasing of total protein concentration which was caused by oxidative stress

    Item Type: Article
    Subjects: R Medicine > RK Dentistry
    Divisions: Faculty of Medicine > Department of Dentistry
    Depositing User: drg Shanty Chairani
    Date Deposited: 08 Aug 2015 01:35
    Last Modified: 08 Aug 2015 01:35
    URI: http://eprints.unsri.ac.id/id/eprint/5305

    Actions (login required)

    View Item