Diagnostic Study of Rapid Test Chlamydia Ag in Patients with Genitalia Infection of Chlamydia trachomatis

Jogoboyo, Yuwono (2013) Diagnostic Study of Rapid Test Chlamydia Ag in Patients with Genitalia Infection of Chlamydia trachomatis. In: 6th International Seminar of Indonesian Society for Microbiology and 11th National Congress of Indonesian Society for Microbiology, 16 - 19 Oktober 2013, Hotel Aston Pontianak.

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    Abstract

    Genitalia infection of Chlamydia trachomatis is a sexual transmitted disease in 10-40% women causing pelvic inflamatory disease (PID) with infertility and ectopic pregnancy complications. This infection is very difficult to diagnose due to clinical asymptomatic manifestations. Some of diagnostic methods were developed but still giving unsatisfied results. One of promising method for detecting C. trachomatis infection is rapid test Chlamydia or point of care test. Gold standard of identification C. trachomatis was culture or detecting nucleic acid by polymerase chain reaction (PCR). This was a diagnostic study conducted in Klinik Graha Sriwijaya Palembang and Departemen Mikrobiologi Klinik RSUP Dr. Moh.Hoesin Palembang from Februari - Mei 2012. Endoservic specimen were collected for Gram staining, rapid test QuickStripeTM Chlamydia Ag (Savyon® Diagnostic Israel), DNA extraction and PCR. The 108 subjects with 26.82 years old in average were recruited. Result of QuickStripeTM Chlamydia Ag were 23 (21,3%) positive and 85 (78,7%) negative. Result of PCR detection were 21 (19,4%) positive and 87 (80,6%) negative. Value of Kappa test between 2 examiners of rapid test QuickStripeTM Chlamydia Ag was 0.893 indicating the examination was reliable. Analysis of rapid test Chlamydia result was compared with PCR result showed sensitivity and spesificity 90,4% dan 95,4% respectively (PPV 82,6%; NPV 97,6%; PLR 19,65%; NLR 0,100). The result was indicated that QuickStripeTM Chlamydia Ag was good for screening of the disease. Conclusion: Rapid Test Chlamydia Ag showed diagnostic significant value and can implementate as a alternative diagnostic method for detecting C. trachomatis infection especially in high risk population. Key Words: Rapid Test Chlamydia Ag, PCR, Diagnostic Study

    Item Type: Conference or Workshop Item (Paper)
    Subjects: R Medicine > R Medicine (General)
    Divisions: Faculty of Medicine
    Depositing User: Yon Yuwono
    Date Deposited: 06 Dec 2013 08:52
    Last Modified: 06 Dec 2013 08:53
    URI: http://eprints.unsri.ac.id/id/eprint/3164

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