Isolation of Antioxidant Compound from Endophytic Fungi Acremonium sp. from the Twigs of Kandis Gajah

Elfita, Elfita and Muharni, Muharni and Munawar, Munawar and Rizki, Rizki (2012) Isolation of Antioxidant Compound from Endophytic Fungi Acremonium sp. from the Twigs of Kandis Gajah. MAKARA SAINS, 16 (1). pp. 46-50. ISSN 1693-6671

[img]
Preview
PDF - Published Version
Download (310Kb) | Preview

    Abstract

    Abstract The endophytic fungi Chrisonilia sitophila, Acremonium sp., and Penicillium sp. have been isolated from the tissues of the twigs of kandis gajah. All of the fungi strains were grown in 3 L potatoe dextrose broth medium (PDB) at a room temperature for 28 days. To extract the antioxidant compounds, the culture broth were filtered for mycelia removal followed by extraction and evaporation. All of the extracts were evaluated for their antioxidant activities by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. The extract of Acremonium sp. have strong activity with IC50 value of 10.3 μg/mL, which is equivalent to ascorbic acid activity with IC50 value of 9.8 μg/mL. The extract was subjected to column chromatography on Si gel twice to obtain a high purity antioxidant compound in the form of yellow oil. The molecular structure was determined based on spectroscopic data, including 1H-NMR, 13C-NMR, HMQC, HMBC, and COSY. The compound was determined as sesquiterpene 3,5-dihydroxy-2,5-dimethyltrideca-2,9,11-triene-4,8-dione.

    Item Type: Article
    Subjects: Q Science > QD Chemistry
    Divisions: Faculty of Mathematics and Natural Sciences > Department of Chemistry
    Depositing User: Dr. Elfita, M.Si
    Date Deposited: 29 Apr 2013 10:02
    Last Modified: 29 Apr 2013 10:02
    URI: http://eprints.unsri.ac.id/id/eprint/2074

    Actions (login required)

    View Item