AMINO ACID SEQUENCE OF B CELL EPITOPE OF N-TERMINAL REGION OF ESAT-6 MYCOBACTERIUM LEPRAE ROLE AS SPECIFIC ANTIGEN FOR DIAGNOSIS OF LEPROSY

Kurdi, Fauziah Nuraini and Handojo, Indro and Agoesni, Indropo and Dahlan, Yoes Priatna AMINO ACID SEQUENCE OF B CELL EPITOPE OF N-TERMINAL REGION OF ESAT-6 MYCOBACTERIUM LEPRAE ROLE AS SPECIFIC ANTIGEN FOR DIAGNOSIS OF LEPROSY. ePrints UNSRI.

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      Abstract

      The objective of this study was to find a specific B-cell epitope of Nterminal region of antigen L-ESAT-6 from leprosy patients, healthy individuals and healthy nurses working for more than 10 years in the leprosy ward of dr.A.Rivai Abdullah Leprosy Hospital, Palembang, Indonesia. Fifty subjects were enrolled in this study, comprising 10 subjects with LL type leprosy, 10 subjects with BB type leprosy , 10 subjects with TT type leprosy, 10 healthy nurses from leprosy ward and 10 healthy individuals as control group. The amino acid sequence of residues 11-36 of the N-terminal region of L-ESAT-6 were divided into a series of 18 peptides each consisting of 9-mer peptides with an overlap of 8-mers and an offset of one amino acid. The series of 18 peptides were synthesized in the form of biotinylated peptides and used to screen sera of 50 subjects using an indirect ELISA method. Our study identified at the N-terminal of L-ESAT-6, LEQCQES, VNELQG and IDALLE as epitope marker for LL and BB type of leprosy, epitope marker for TT type of leprosy and for protective epitope marker) healthy nurses working for more than 10 years in the leprosy ward, respectively. These antigens can be used in immunochromatographic test for the early diagnosis of leprosy.

      Item Type: Article
      Additional Information: Physical and Health Study Program, Teacher Training and Education Faculty and Rehabilitation Medicine, Medical Faculty, Sriwijaya University, Palembang; Department of Clinical Pathology, Department of Dermatology and Venerology, Department of Parasitology, Medical Faculty, Airlangga University, Surabaya, Indonesia
      Uncontrolled Keywords: L-ESAT-6, B-cell epitope mapping, M. leprae diagnostic tool, protective marker, leprosy marker
      Subjects: R Medicine > R Medicine (General)
      Divisions: Faculty of Teacher Training and Science Education > Study Program of Sport and Health Education
      Depositing User: Candra Setiawan
      Date Deposited: 16 Jan 2012 22:33
      Last Modified: 29 Feb 2012 17:41
      URI: http://eprints.unsri.ac.id/id/eprint/176

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