PURIFIKASI DAN KARAKTERISASI PROTEASE DARI BAKTERI PATOGEN Pseudomonas aeruginosa

Baehaki, Ace and Suhartono, Maggy T and Palupi, Nurheni Sri and Nurhayati, Tati (2008) PURIFIKASI DAN KARAKTERISASI PROTEASE DARI BAKTERI PATOGEN Pseudomonas aeruginosa. Jurnal. Teknol. dan Industri Pangan, XIX (1).

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    Abstract

    In the last decade, a concern on protease as medicinal target for overcoming bacterial diseases and viral diseases has been rapidly increased because of the obvious involvement of this enzyme in the molecular of the diseases mechanism. The purpose of this research was to purification and characterize protease from patogenic bacteria Pseudomonas aeruginosa. The bacteria were grown in media containing triptone 1%, NaCl 1% and Yeast extract 0,5%. Protease of P.aeruginosa was purifed using column chromatography with Sephadex G-100 gel. There were three peaks of enzyme protein, which were detected on fractions 14, 17 and 30. The optimum pH of the extracellular protease from P. aeruginosa was 8.. The optimum temperature of P. aeruginosa protease was 300C. Fe3+ (1 dan 5 mM) was strong activator and Co2+ was strong inhibitor. Study on the effect of metals ion and spesific inhibitors indicated that protease from P. aeruginosa was serin metaloprotease. The apparent moleculer weights, as determined by SDS-PAGE and zymogram technique,36 kD and 42 kD.

    Item Type: Article
    Uncontrolled Keywords: Protease, characterization, purification, patogenic bacteria P.aeruginosa
    Subjects: S Agriculture > S Agriculture (General)
    Divisions: Faculty of Agriculture > Department of Agriculture Product Technology
    Depositing User: Candra Setiawan
    Date Deposited: 10 Dec 2011 13:06
    Last Modified: 28 Dec 2011 01:09
    URI: http://eprints.unsri.ac.id/id/eprint/11

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