ISOLASI DAN KARAKTERISASI PROTEASE DARI BAKTERI TANAH RAWA INDRALAYA, SUMATERA SELATAN

Baehaki, Ace and A, Rinto and Budiman, Arief (2011) ISOLASI DAN KARAKTERISASI PROTEASE DARI BAKTERI TANAH RAWA INDRALAYA, SUMATERA SELATAN. J. Teknol. dan Industri Pangan, 22 (1).

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    Abstract

    In an effort of obtaining indigenous protease producing bacteria, screening bacterial protease was conducted from samples collected from Indralaya soil swamp, South Sumatera. Three of 31 colonies showed high protease activity with proteolytic index >1.00. T1S1 produced enzyme with the highest activity. The crude enzyme activity after 48 hours incubation was 0.391 IU/ml. The optimum pH of the extraceluller proteases from T1S1, T3S2 and T3S3 were 8.0, 8.0, and 7.5, respectively. The optimum temperature of T1S1, T3S2 and T3S3 proteases were 40, 50, and 500C, respectively. All metal ions tested (Na+, K+, Mn2+, Zn2+ and Fe2+) inhibited proteases except Fe2+ which activates the T3S3 protease at 5 mM. EDTA (1 and 5 mM) inhibited all proteases. Study on the effect of metals ion and spesific inhibitors indicated that all protease are metaloprotease. Moleculer weights was determinited using SDS-PAGE and zymogram technique. The moleculer weight of T1S1 protease was 121 kD, whereas for T3S2 protease were 51, 71, and 119 kD and T3S3 protease were 49, 70, and 116 kD.

    Item Type: Article
    Uncontrolled Keywords: Protease, isolation, characterization, bacteria
    Subjects: S Agriculture > S Agriculture (General)
    S Agriculture > SH Aquaculture. Fisheries. Angling
    Divisions: Faculty of Agriculture > Department of Fishery Products Technology
    Depositing User: Candra Setiawan
    Date Deposited: 09 Dec 2011 16:13
    Last Modified: 31 Jan 2012 22:01
    URI: http://eprints.unsri.ac.id/id/eprint/10

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